Blot flag in Mobi

DocHub enables users to blot flag in Mobi digitally

With DocHub, you can quickly blot flag in Mobi from any place. Enjoy capabilities like drag and drop fields, editable text, images, and comments. You can collect electronic signatures safely, include an extra level of defense with an Encrypted Folder, and collaborate with teammates in real-time through your DocHub account. Make changes to your Mobi files online without downloading, scanning, printing or mailing anything.

Follow the steps to blot flag in Mobi files online:

Click New Document to upload your Mobi to your DocHub account.
View your document in the online editor by clicking Open next to its name. If you prefer, click on your file instead.
blot flag in Mobi and make more changes: add a legally-binding eSignature, include extra pages, insert and remove text, and apply any tool you need from the top toolbar.
Use the dropdown menu at the very right-hand top corner to email, download, or print your file and send it for signing.
Transform your document to reusable template.

You can find your edited record in the Documents folder of your account. Create, email, print out, or turn your document into a reusable template. With so many powerful tools, it’s simple to enjoy seamless document editing and management with DocHub.

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Smooth teamwork

Collaborate on documents with your team using a desktop or mobile device. Let others view, edit, comment on, and sign your documents online. You can also make your form public and share its URL anywhere.

Automatic saving

Every change you make in a document is automatically saved to the cloud and synchronized across all devices in real-time. No need to send new versions of a document or worry about losing information.

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How to blot flag in Mobi

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Got questions?

Below are some common questions from our customers that may provide you with the answer you're looking for. If you can't find an answer to your question, please don't hesitate to reach out to us.

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What are FLAG-tagged antibodies?

Anti-FLAG antibodies are reagents that can be used to detect or stain Flag-tagged recombinant proteins. The FLAG epitope tag, is an octapeptide containing the amino acid sequence DYKDDDDK.

What are the tags for western blot?

Peptide tags, such as HA (Hemagglutinin), Myc, DYKDDDDK, and V5 are popularly used for detection in western blot, immunocytochemistry, and co-immunoprecipitation.

How do you elute FLAG-tag protein?

The FLAG-tagged protein binds to the FLAG-tag specific monoclonal antibody conjugated on an agarose gel. After washing away residual impurities, bound FLAG-tag proteins can be eluted off the affinity column by high concentration of the FLAG-tag peptide or by low pH buffer.

What is the FLAG-tag in Western blot?

The Flag-tag is either N- or C-terminally cloned to the protein of interest. After expression, the recombinant Flag-tag fusion protein can be analyzed by means of ELISA, flow cytometry, immunofluorescence (IF), immunoprecipitation (IP) co-immunoprecipitation (Co-IP), protein purification, and Western blotting (WB).

How many kDa is a FLAG tag?

As its second name suggests the tag consists of an amino acid sequence DYKDDDDK. (D=Aspartic acid; K=Lysine; Y=Tyrosine). That brings the total size of the tag to 1012.9 dalton or roughly 1 kDa (1).

What is flag in plasmid?

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a peptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine).

What is the use of FLAG tag?

The FLAG tag has major application in protein purification from mammalian expression systems or cell lines and in studies like immunoprecipitation, immunofluorescence (for protein localization), ELISA, immunostaining, SDS-PAGE protein electrophoresis (for protein detection), flow cytometry, or western blotting assays.

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