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Western blot analysis is a powerful method of detection, because antibodies that target specific protein epitopes are used to probe for your proteins of interest. The most common Western blot strategy to detect specific proteins is indirect detection, in which the target-specific antibody, called the primary antibody, binds to the protein of interest, and then a second antibody labeled with a detectable enzyme or fluorophore binds to the primary antibody. Unlike other methods, indirect detection allows multiple secondary antibodies to bind to each primary antibody, resulting in signal amplification. Indirect detection strategies use antibodies made in different host species in order to restrict antibody binding to the intended target. Lets say you want to probe a human cell lysate for actin, and your primary antibody is a mouse anti-actin antibody, which means that the antibody was collected from a mouse and specifically binds to actin. You look in your labs secondary antibody box an
