Blot flag in doc

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Aug 6th, 2022
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How to blot flag in doc

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foreign letamp;#39;s go time to play some soccer well I understand itamp;#39;s important to take some rest when your body isnamp;#39;t supporting but itamp;#39;s also important to take needed measures and overcome hurdles as Cristiano Ronaldo did sure so in todayamp;#39;s episode let us get inspired by the story of Cristiano Ronaldo zoom in on the morning of February 5th 1985 a baby boy was born in Madeira Portugal and was named Cristiano Ronaldo dos Santos abiero but instead of celebrating Cristianoamp;#39;s parents were unhappy about it due to their poor living conditions and lack of resources for raising a fourth child so poor Cristiano grew up in a humble background with such a tight Financial State that he and his friends often visited the local Burger Shop to ask for leftover food but the vital aspect he and his friends shared was the love for football as they spent most of their time playing the spirited game on the streets of their town with a ball made of

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FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a peptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine).
The FLAG tag is a short peptide (AspTyrLysAspAspAspAspLys) that is bound by a specific antibody. Anti-FLAG antibody is available commercially and may be attached to a suitable resin for use in column purification. The Strep tag is a 10 amino acid peptide that mimics the 3D structure of biotin.
Typically the Flag-tag is used for protein purification from mammalian expression systems or general immunostaining and immunoprecipitation assays.
The Flag-tag is either N- or C-terminally cloned to the protein of interest. After expression, the recombinant Flag-tag fusion protein can be analyzed by means of ELISA, flow cytometry, immunofluorescence (IF), immunoprecipitation (IP) co-immunoprecipitation (Co-IP), protein purification, and Western blotting (WB).
As already mentioned the Flag-tag can be fused either to the C-terminus or the N-terminus of a protein of interest. Which terminus is better suited for the fusion is protein-dependent and needs to be tested for each protein individually.
Anti-FLAG antibodies are reagents that can be used to detect or stain Flag-tagged recombinant proteins. The FLAG epitope tag, is an octapeptide containing the amino acid sequence DYKDDDDK.
The FLAG tag has major application in protein purification from mammalian expression systems or cell lines and in studies like immunoprecipitation, immunofluorescence (for protein localization), ELISA, immunostaining, SDS-PAGE protein electrophoresis (for protein detection), flow cytometry, or western blotting assays.

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