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hi everyone thanks for joining us for our Western blot webinar today weamp;#39;ll be going through the basic principles of the Western blot and Iamp;#39;ll show some examples of troubleshooting this technique remember that if you have any questions please submit them to the Qamp;amp;A panel on the right hand side of your screen and Iamp;#39;ll be available to answer some of them at the end of our presentation so what is a Western blot and how does it work itamp;#39;s a complicated technique but to summarize itamp;#39;s used to identify a specific protein of interest within a sample containing many proteins first the proteins are separated from each other ing to molecular weight using gel electrophoresis and then a specific antibody is used to identify the protein of interest so we can determine the molecular weight of the protein of interest by comparing the results to protein standards of known molecular weight and we can also determine the relative quantity of the protein of in
