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in this video you will see how a Western transfers perform using the bolt mini block module one tank make sure cassette clamps are removed before you set up your transfers one gel is transferred per module and two modules fit in one bolt tank included in the bolt block module kit is a roller sponge pads tweezers and the quick reference card approximately 250 milliliters of transfer buffer is required for each transfer prepare transfer buffer by adding 50 milliliters of 20 X bolt transfer buffer 100 milliliters of methanol and one milliliter of anti oxidant bringing up the total volume to one liter with deionized water so 2 sponge pads in 1x transfer buffer and squeeze the pads to remove air bubbles prepare the gel for transfer by first rinsing the gel cassette with water open the gel cassette using a gel knife carefully remove the wells and the foot of the gel using the gel knife so that the entire gel is of equal thickness place a shallow dish on the bench and pour 1x transfer buffer
