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in this video youamp;#39;ll learn how to transfer protein from polyacrylamide gels to membranes using the Invitrogen I bought two dry blotting system the eye blot - dry blotting system uses preassembled transfer stacks with integrated nitrocellulose or PVDF membranes offering the convenience of a bufferless plug-and-play system I blot two stacks consists of a copper cathode and anode sandwich and rye gel matrices containing the appropriate buffers so there is no need to prepare transfer buffer both mini and regular blots decks are available mini blot stacks transfer 1 mini blot while regular blot stacks can transfer one middie blot or up to two mini blots to get started turn on the eye blot to dry transfer device and open the lid the digital display will show icons for the available actions next unseal the transfer stack notice that the top and bottom stacks are in the same tray separate the top stack and set it aside keep the bottom stack in the plastic tray the bottom stack contains