Many people find the process to blot expense in UOML quite challenging, especially if they don't often deal with paperwork. Nevertheless, these days, you no longer have to suffer through long tutorials or spend hours waiting for the editing app to install. DocHub enables you to adjust documents on their web browser without setting up new applications. What's more, our feature-rich service provides a full set of tools for comprehensive document management, unlike numerous other online solutions. That’s right. You no longer have to export and import your forms so often - you can do it all in one go!
No matter what type of document you need to alter, the process is easy. Make the most of our professional online solution with DocHub!
in this video we will discuss considerations for optimizing incubation time and dilution of primary antibodies when performing a western blot commercially purchased antibodies should have a starting dilution recommended by the manufacturer plan your dilution series around the manufacturer recommended dilution for example if a one to one thousand dilution was recommended make and test a two-fold dilution series ranging from a one to two hundred and fifty dilution to a one to four thousand dilution this should help establish the optimal dilution for your specific target protein the optimal primary antibody dilution has been established you can optimize incubation time the typical recommendation for primary antibody incubation is one to two hours at room temperature or overnight at 4 degrees celsius the optimal choice depends on your antibody and sample for example if the target protein is abundant in your sample a 1 to 2 hour incubation at room temperature
