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hi in this video weamp;#39;re looking at how to normalize your western blot data. so it includes the recommendation from the journal of biological chemistry (JBC) so letamp;#39;s get into it youamp;#39;ve electrophoresed the proteins down a gel, youamp;#39;ve placed the gel on a nitrocellulose or pvdf membrane and transferred the protein from the gel to the membrane so that you can probe it with a specific antibody now where you detect differences in the band intensities for the different lanes that you probe with antibody which represents different experimental conditions how do you know that the differences that youamp;#39;re seeing are real as in they are biologically determined rather than maybe as a result of the technique that youamp;#39;ve used this is where normalization comes in what is normalising? normalising is used in experiments a lot because what you want to do is to confirm that the variations that you see if youamp;#39;re seeing varia
