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welcome to part two of how to master the technique of Western plotting I will show you in detail how to assemble a semi driver some blood sandwich please donamp;#39;t forget to check out my personal blog where you can find a detailed written protocol of the following video content letamp;#39;s Dive Right In first I cut Woodland paper and Nitro cellulose membranes by hand it is cheaper to cut them on your own another Advantage is the possibility to adjust the size and calculate the proper and purple area of your blood next I ride with a pen the name of the experiment and the date on the membrane I take out the pre-chilled semi-dried transfer buffer and pour a little amount into three individual containers where I incubate the membrane the what my papers and the SDS gel but first I need to activate the Nitro cellulose membrane in water be aware that a pvdf membrane must be activated in methanol next I take the SDS gel and incubate it in transfer buffer foreign spatula a
