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Dot blot is a quick method for detecting biological samples like proteins or nucleic acids, functioning similarly to western and southern blotting but dotting samples directly onto a membrane for detection. Its simplicity allows for diverse applications, including monitoring labeling efficiency, estimating protein concentrations, and comparing antibody performance. For instance, to determine labeling efficiency with a biotin-labeled sample, one would compare it to a known biotinylated reference standard, making serial dilutions of both samples in the same manner, with a 10-fold dilution providing a wide range of samples.
