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Western blotting is a key technique used to confirm the presence of specific target proteins in a sample. The process begins with SDS-PAGE, where proteins are denatured and negatively charged, allowing them to migrate towards a positively charged anode based on their molecular weight. After electrophoresis, the polyacrylamide gel is stained to visualize protein bands. Marker proteins serve as references to estimate the molecular weight of the sample proteins. However, the presence of a band at a certain molecular weight, such as around 60 kilodaltons, does not conclusively identify the specific proteins present, including the protein of interest within that range.