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welcome back today well be talking about the blotting tests and I try to cover everything that youll need to know for you step 1 exam before we jump into the mechanism youll have to know that we use the blotting test to detect the presence of certain segments of DNA or detect the presence of certain gene expression or the presence of a specific protein and this is how this is done so well start off on the sample of DNA that may or may not contain the certain segments of DNA that you are looking for well use cleaving enzymes to take off one strand of the DNA and well add a radio-labelled primer that is specific to that DNA that we want for example if were looking for HIV DNA well add label primers to that HIV DNA if that sample of DNA did in fact contain some HIV DNA this primer very specific for that HIV DNA will link to this segment and then we will be able to detect it now the process of detecting a specific protein is a little bit different we start off with a sample that ma