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Dot blot is a straightforward method for detecting biological samples such as proteins or nucleic acids, similar to western and southern blotting but without transferring the sample from a gel. Instead, samples are directly dotted onto a membrane for detection. Due to its simplicity, dot blot is versatile and used for various applications, including monitoring labeling efficiency with different probes, estimating specific protein concentration, and comparing antibody performance. For instance, to assess labeling efficiency of a biotin-labeled sample, one would compare it to a known biotinylated standard by making serial dilutions of both, with a 10-fold serial dilution providing a wide range for analysis.
