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Dot blot is a straightforward method for detecting biological samples such as proteins or nucleic acids, similar to western and southern blotting but without the need for gel transfer. Instead, samples are directly dotted onto a membrane for detection. Due to its simplicity, dot blot is versatile and is used in various applications, including monitoring labeling efficiency, estimating protein concentration, and comparing different antibodies. For instance, to determine labeling efficiency with a biotin-labeled sample, serial dilutions of the test sample and a known biotinylated reference are made in the same way, starting with a 10-fold serial dilution to provide a comprehensive range of samples.
