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Dot blot is a quick and straightforward method for detecting biological samples, such as proteins or nucleic acids. It operates similarly to western and southern blotting; however, the samples are directly dotted onto a membrane rather than being blotted from a gel. Due to its simplicity, dot blot is versatile and can be used for various applications, including monitoring labeling efficiency with probes, estimating protein concentration in samples, and comparing antibody performances. For instance, to determine labeling efficiency with biotin, one would compare a biotin-labeled sample to an established biotinylated reference standard by making serial dilutions of both in the same manner, starting with a 10-fold dilution for a wide range of samples.