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In this video tutorial, the dot blotting technique is discussed as a method for identifying the presence or absence of biomolecules such as DNA, RNA, or proteins in tissue, cells, or samples. It is a simplified version of Southern, Northern, or Western blotting, and its main advantage is that it does not require electrophoresis or the separation of biomolecules by size. Consequently, there is no need to transfer biomolecules from an electrophoretic gel to a membrane. Instead, biomolecules are directly added as dots onto the membrane. The tutorial shows how to place different biomolecular samples, like proteins, onto the membrane for analysis.
