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Hello friends today in this video Im going to discuss about the dot blotting dot blotting technique is used for the identification of presence or absence of biomolecules like DNA RNA or protein in a tissue or cell or in a sample okay and this dot blotting technique is simplified form of Southern blood Northern blood or Western blood and its Simplicity is its advantage okay and it is called the simple process because here no electroforesis is required that means no separation of biomolecules ing to their size is required okay so here not so as we omit the electroforesis step so we dont require to transfer the biomolecules from the electrophoric gel to the membrane okay so we have to add the biomolecules directly on the membrane okay that is a direct blotting on membrane it is done like this suppose this is the membrane okay and different biomolecular samples for example protein samples are added to the membrane like do as a DOT okay suppose these are the different protein samples adde