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In this video tutorial, the dot blotting technique is introduced as a method for identifying biomolecules like DNA, RNA, or proteins in tissues, cells, or samples. It is a simplified version of Southern, Northern, and Western blotting, with the main advantage being its ease of use. Unlike the other techniques, dot blotting does not require electrophoresis, meaning there is no need to separate biomolecules by size or transfer them from a gel to a membrane. Instead, biomolecular samples are directly applied to the membrane as dots, facilitating a straightforward process for detecting the presence or absence of the target biomolecules.
