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Dot blot is a straightforward method for detecting biological samples such as proteins or nucleic acids, similar to western and southern blotting, but involves dotting samples directly onto a membrane. Its simplicity allows for diverse applications, including monitoring labeling efficiency, estimating protein concentration, and comparing antibody performance. For instance, to assess labeling efficiency of a biotin-labeled sample, one would compare it to a known biotinylated reference standard. This involves creating serial dilutions of both the test sample and reference standard in the same manner, with a 10-fold dilution being a recommended approach for a broad sample range.
