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Dot blot is a straightforward technique for detecting biological samples such as proteins or nucleic acids, resembling western and southern blotting but directly applying samples onto a membrane instead of transferring them from a gel. Its simplicity makes it suitable for various applications, including monitoring labeling efficiency, estimating protein concentration, and comparing antibody performance. For instance, to assess labeling efficiency with biotin, one should compare the labeled sample against a known biotinylated reference standard by performing serial dilutions of both. Using a 10-fold serial dilution provides a broad range of samples for effective analysis.
