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In this video, the dot blotting technique is discussed as a method for identifying the presence or absence of biomolecules such as DNA, RNA, or proteins in tissues, cells, or samples. This technique is a simplified version of Southern, Northern, or Western blotting, and its main advantage lies in its simplicity. Unlike other methods, dot blotting does not require electrophoresis for size-based separation of biomolecules. Instead, biomolecules are directly applied as dots onto a membrane, eliminating the need for transferring them from an electrophoretic gel. The process allows for straightforward application of various biomolecular samples onto the membrane.
