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Dot blot is a quick and simple technique for detecting biological samples such as proteins or nucleic acids, analogous to western and southern blotting, but with samples applied directly onto a membrane without gel transfer. Its simplicity allows for diverse applications, including monitoring labeling efficiency, estimating protein concentration, and comparing antibody performance. For instance, to assess labeling efficiency using a biotin-labeled sample, one can compare it to a known biotinylated reference standard. This involves making serial dilutions of both the test sample and reference standard, ensuring identical dilution methods, starting with a 10-fold dilution for a broad range of samples.
