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Western blotting is the leading method for protein detection and characterization. While its basic principles remain unchanged, journal standards for publishing Western blots have become increasingly rigorous. Understanding both the theory and execution of Western blotting is crucial for obtaining accurate protein quantification. To manage variability and enhance result accuracy, it’s essential to maximize both accuracy and precision for meaningful relative comparisons. This can be achieved by limiting inherent sample and method variability through careful experimental design, thereby minimizing necessary corrections during normalization. Additionally, employing the right internal loading control is vital for effective normalization. Ensure that your Western blot data complies with current publication standards.