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hello everyone in this video Im going to speak about blotting techniques which are widely used techniques um in the biochemistry proteomics um and even in molecular biology of course in this video Im going to speak about Western blotting there are also other blotting techniques so Western blotting is specifically used to detect proteins Southern blotting is used to detect DNA and Northern blotting to detect RNA in this video Im going to speak about Western bloating and there is another video about southern blotting and Northern blotting so lets start speaking about a western blotting technique of course Western blotting is a technique used to detect proteins so its used to detect a specific protein in a protein mixture lets say that I that I have a protein extract from my cell a prokaryotic cell or an eukaryotic cell whatever and I have a protein mixture and I am interested in the to detect a specific protein in this mixture what I have to do is to use Western blood the thing is