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Dot blot is a quick method to detect biological samples like proteins or nucleic acids, similar to western and southern blotting, but applies samples directly onto a membrane instead of from a gel. Its simplicity allows for various applications, including monitoring labeling efficiency, estimating protein concentration, and comparing antibody performance. For instance, to determine labeling efficiency with a biotin-labeled sample, one can compare it to a known biotinylated reference standard. This involves making serial dilutions of both the test sample and reference standard in the same manner, with a 10-fold serial dilution recommended for a broad range of samples.
