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In this tutorial, the dot blotting technique is discussed as a method for identifying the presence or absence of biomolecules such as DNA, RNA, or proteins in tissues or samples. This technique is a simplified version of Southern, Northern, or Western blotting, with its key advantage being its simplicity. Unlike other blotting methods, dot blotting does not require electrophoresis, so there is no need to separate biomolecules by size or transfer them from a gel to a membrane. Instead, biomolecules are directly applied to the membrane in a dot formation, making the process straightforward and efficient.
