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Dot blot is a method for detecting biological samples like proteins or nucleic acids. It is similar to western blotting and southern blotting, but samples are dotted directly onto a membrane. This method is simple and can be used for various applications such as monitoring labeling efficiency, estimating protein concentration, and comparing antibody performance. To determine labeling efficiency of a biotin-labeled sample, compare it to a known biotinylated reference standard by making serial dilutions of both samples in the same way. A 10-fold serial dilution is recommended for a wide range of samples.
