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A dot blot is a method for detecting biological samples by directly dotting them onto a membrane. It is similar to western blotting and southern blotting but simpler. It is used for various applications like monitoring labeling efficiency, estimating protein concentration, and comparing antibody performance. To determine labeling efficiency, compare a labeled sample with a known biotinylated standard through serial dilutions in the same way. A 10-fold dilution is recommended for a wide range of samples.