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Today, we are discussing the dot plot hybridization technique used to detect specific DNA or RNA sequences through electrophoresis. This technique involves southern blotting for known fragments and northern blotting for new fragments. Hybridization with a probe forms a double-stranded DNA, allowing for easy identification. Steps include purifying DNA or RNA samples, applying them to a nitrocellulose or nylon membrane, denaturing the DNA, and fixing it for analysis.
