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Today we are discussing the dotplot hybridization technique, which is used to detect specific DNA or RNA sequences in a sample. This technique involves southern blotting for known fragments and northern blotting for new fragments. The key principle is hybridization, where a probe binds to the target DNA, forming double-stranded DNA. The steps in the dot blot technique include purifying DNA or RNA samples, applying them to a nitrocellulose or nylon membrane, denaturing the DNA, and fixing it in place. Nitrocellulose membrane is preferred for its affinity for nucleic acids. This technique helps in identifying specific DNA fragments easily.
