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Today's discussion is on the dot blot hybridization technique. This technique is used to detect specific DNA or RNA sequences in a known sample through electrophoresis. The procedure involves southern blotting for known fragments and northern blotting for new fragments, with hybridization being the key principle. The probe binds to the target DNA, forming a double-stranded DNA. Steps include purifying DNA or RNA samples, applying them to a nitrocellulose or nylon membrane, denaturing the DNA, and fixing it. Nitrocellulose membrane is preferred for its affinity for nucleic acids. The probe is radio labeled for easy identification.