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Dot blot hybridization is a technique used to detect specific DNA or RNA sequences in a sample. It involves southern blotting for known fragments and northern blotting for new fragments. The principal is hybridization, where a probe binds to the target DNA, forming double-stranded DNA. Steps include purifying DNA or RNA, using a nitrocellulose or nylon membrane, applying samples, denaturing DNA, and fixing it by binding the probe. This technique is essential for identifying specific DNA fragments easily.
