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today the topic of our discussion is what is dotplot hybridization technique a very short summarized video why we need dotplot technique its a technique that is used to detect the presence of a specific sequence of DNA or RNA in a known electrophoresis to the sample normally the procedure what we do to find out a specific DNA fragment is southern blotting and for our new fragment it is northern blotting and the principal is hybridization the probe will bind to the target DNA and forming a double-stranded DNA so that as the probe is radio labeled we could identify the probe easily lets move into the detail the steps in dot blot technique first of all we need to purify either DNA or RNA of different samples as per our requirement then this is a nitrocellulose membrane or a nylon membrane at aussolas membrane is preferred as it has more affinity for nucleic acids so we need to apply the DNA or RNA samples the stores just like this so denature DNA as it is double-stranded and fix it by
