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welcome again friends we talking about uh blottings now in this video weamp;#39;ll be talking about the Western blotting now Southern blotting is applied for the detection of DNA Northern blotting is utilize for the detection of RNA and Western blotting is utilized for the detection of proteins this is also called imuno blotting because in this case weamp;#39;ll be seeing that antigen and antibody interaction can be utilized beautifully uh to get our results now what are the application and advantages now the applications for this blotting is that uh verify the expression of a protein inside the cell we can get it second thing is that we can determine the relative amount of a protein present in different samples so if we get different samples from the different time limit of a different time Gap from the same cell we can get the protein expression or amount of protein expression present in different time from from the cell and also we can analyze the protein protein interacti