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northern blood is a classic technique in molecular biology that reveals information about rna identity size and abundance and it can be used to study gene expression by detection of isolated mrna in a sample the first step in a northern blot is the preparation of the rna samples during this step formaldehyde is added to the samples for the denaturation of the rna molecules rna typically is a single stranded nucleic acid however the presence of self-complementary sequences in the rna strand leads to intrachain base pairing and folding of the ribonucleotide chain into complex structural forms known as secondary structures formaldehyde contains a carbonyl group that reacts to form shift bases with the amino or amino groups of nucleobases these covalent adducts prevent normal base pairing and maintain the rna in a denatured state after preparation of the samples the rnas are separated by gel electrophoresis for the separation a loading buffer is added to the samples and used as a tracking
