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so now weamp;#39;re going to see examples of how Western blots are used by scientists to quantitate protein levels and so Iamp;#39;ve taken a random figure out of a paper I just want to show you how scientists use Western blocks so if you look in this block on the left letamp;#39;s number the lanes so you can see what Iamp;#39;m referring to leans one two three four five and six and usually above the land is written something that indicates how the samples were treated so if you looked above the lanes there are terms like DMSO 0.73 0.73 and above that it says four hours and those are in Lanes one two and three and then next to its 24 hours and youamp;#39;ll get to learn about the nomenclature as we read more and more papers in this instance they are adding drugs to a cell increasing concentrations of drugs and testing the protein levels at 4 hours and 24 hours a post to treatment of drugs DMSO is the negative control that is an organic solvent that drugs are dissolved in so in Lan