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Iamp;#39;m going to show you how to quantify Western blots so the first step after Imaging is youamp;#39;re going to select the file that the images are saved on right here this is the file Iamp;#39;ll be selecting once youamp;#39;ve opened up the file youamp;#39;re going to see all these different documents we want to select the mscn documents these will be the mscn images these are the images produced from the Multi Image so we open it up and it will automatically open up with image lab software the program now we have three different blots in front of us we are going to be using the chemiluminescent blot so we select it and we go over to the left to Lane and bands under Lanes we see Lane finder and we can go to manual now you can select how many lanes you want we could increase or decrease but weamp;#39;re going to stick with four today press ok and four lanes will automatically pop up we want to take the corners and drag it so that we can perfectly or near perfectly fit our b