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hi everyone and Welcome to our Channel Tipsy pipette where we solve your day-to-day research problems in todayamp;#39;s video we will show that after doing 2-3 days of exhaustive Western blotting how you guys can properly quantify your Bloods and later plot in any graphing software for the quantification purpose we will be using a freely available software called image developed by NIH for which the download link and installation instruction is provided in the description below so letamp;#39;s get started as we previously showed this here is a clean block showing distinct bands with different expression levels letamp;#39;s open image J software and you can drag your file in it which will open the image then select the rectangle tool and draw a Box covering the first band nicely make sure to make the Box vertically longer otherwise while quantification it will give error after making the Box press 1 then drag the box to the next band and press 2 then again drag the box and press 2 re
