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so you want to start carrying out for us in Western blots because now youamp;#39;ve heard all the flexibility that it gives to your procedure but youamp;#39;re not quite sure yet how to go about collecting antibodies for your turn Iamp;#39;m here to help you with them whether they walk fly or crawl you have to consider whole species for outdoor activity and select antibodies ingly one exception is when the primary antibody itself is a human recombinant antibody directly tagged with a fluorescent molecule in that case those can be paired with any antibody from any species to be able to carry out multiplex analysis the excitation and emission spectra of the fluorophores you pick should not overlap pick that one when carrying out fluorescence Western blotting for one or multiple proteins always choose the secondary antibody with a brightest flora for such as fire red star bright die tip number four be aware of the excitation laser and emission filters in your fluorescence imager the im
