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[Music] in this video we will discuss the key criteria you should consider when choosing between nitrocellulose and pvdf membranes for your western blinding experiment when it comes to western blotting choosing the best membrane for your application can mean the difference between crystal clear bands and a blotchy mess there are four key criteria you should consider when choosing a western blotting membrane the first criterion you should consider is protein binding capacity in general pvdf has a higher protein binding capacity than nitrocellulose this means pvdf is more sensitive but also more prone to background noise choose pvdf for applications requiring high sensitivity next you should consider pore size both membrane types are available in a variety of pore sizes ranging from 0.1 to 0.45 microns although 0.45 microns is suitable for most protein applications 0.1 or 0.2 microns is recommended for proteins or peptides less than 15 kilodaltons in size also consider each membranes du