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revolutionize your western workflow with easy west light genscripts western blotting device that automatically completes the process of blocking antibody incubation and washing steps with many advantages including automatic antibody recovery and a customizable program easy west delivers consistent results on the side of the instrument pull out the cartridge and load the blocking buffer primary antibody and secondary antibody reagents the minimum volume requirement for each reagent is 10 ml once the reagent is loaded press the cap firmly on the back of the device it is attached to a bottle holding the wash buffer and a second bottle for collecting the waste reagent the user-friendly touch screen interface allows customization of the western protocol once all reagents are loaded take out the cassette for loading the membrane open the cassette and note the markings on the surface this includes the placement position of the membrane and the spacers align the membrane with bands facing up