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all right letamp;#39;s learn how we can quantit quantified bands using image j start image j now weamp;#39;re going to open up a gel file say file open and itamp;#39;s in the same folder here this urease purification number two alright hereamp;#39;s our hereamp;#39;s our gel that will will practice quantifying bounce relatively toward their lane so what weamp;#39;ll do is weamp;#39;ll first figure out what is the general signal for the lane do this first we need to invert the image so that the bands are now white and the background is black the reason for this in most image analysis black is equal to zero and color or white is equal to some value okay so letamp;#39;s get a value a numerical value for a lane number two weamp;#39;re gonna use this box tool weamp;#39;re gonna draw a box around the lane weamp;#39;ll try to make it fit fairly well around the lane okay and now weamp;#39;ll go to analyze and will say set measurements and weamp;#39;re gonna uncheck everything exce