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WESTERN BLOTTING PART III: Transfer, Blotting and Visualization. To process the gel further for the detection and visualization of specific proteins and/or molecules, the proteins are transferred from the gel, onto a more durable material that can be manipulated and blotted with antibodies of interest. Transferring is necessary to allow antibodies free access to proteins and because polyacrylamide gels are fragile and difficult to manipulate. The transfer method most commonly used in western blotting is electroelution, where an electric field is applied to the gel to facilitate the migration of the proteins out of the gel and onto a piece of membrane. Transfer is accomplished by placing the polyacrylamide gel in direct contact with a membrane made of nitrocellulose or another non-reactive material that binds to proteins and submerging both in a buffer-filled chamber. The gel/membrane sandwich is placed in such as way that the negative electrode is located closest to the gel while the p
