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reverse phase protein array or rppa is an excellent method to quantitate proteins and phosphorylates in complex protein lysates such as cell or tissue lies aids my name is Cristina short I have been working with rppa since many years at the Institute of Pathology at the Technische universitt mnchen Germany in this video we will show you how to perform a typical are TPA experiment we will start with a protein lysate this lysate can be prepared from cells animal tissues or human tissues then I will show you why and how to generate dilutions from this lies 8 using an automatic liquid handling system after that I will demonstrate how to spot the diluted protein lysates onto nitrocellulose coated glass slides with the help of a robotic area then I will show you how validated antibodies are used to detect the proteins or forceful proteins of interest finally Katerina will explain how to analyze the data first lysate you can use any protein lysate from cells animal or human tissues to gener
