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are your western blots interrupting your busy schedule do you feel like youamp;#39;re babysitting your blots have you ever lost track of where you are in the protocol compromising ideal results if so check out the ibine western system for your next western the ibind western system performs all immunodetection steps in a seamless sequential process blocking primary antibody secondary antibody and wash steps are sequentially automated in a fully walk away technique the ibine western system comes with an eyebind device eyebind cards an ibine solution kit and a roller the first step is to prepare the 1x iobine solution to begin add 23.7 milliliters of deionized water into a conical tube then add six milliliters of the ibine buffer followed by 300 microliters of the eye bind additive then swirl the mix to prepare the run immerse your blotted membrane in 5 milliliters of the 1x ibind solution next dilute your antibodies with the 1x ibind solution ing to the protocol recommendations
