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if youamp;#39;ve been in a biochemistry lab you might be all too familiar with this thing yep weamp;#39;re talking western plotting so western blotting is this technique that we can use to look for specific proteins after we do an sds page gel today is like a more casual um like what does it actually look like how do we set things up type of thing um introduction to western blotting i previously did a longer um video on the i mean like a video with more of the background and more graphics um for people um and so it might be a better place to start if youamp;#39;re not familiar with western blotting and so i will link to that in the comments as well as to text versions so that you have all the opportunities to learn more about this technique that i hate so an sds page gels basically where we take a little bit of a sample containing some proteins and we run it through this gel mesh and itamp;#39;s going to separate by size so basically we take the proteins and we um denature them so