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does your next experiment involve tgf beta 1 this video is for scientists who want to run western blot ihc flow or other immunoassays against tgfb1 we will cover some basic facts protein expression and tgf beta 1amp;#39;s expected behavior in western blot all information in this video is based on public information and no proprietary experimental evidence was used topic one quick facts about tgf beta 1. transforming growth factor beta 1 is part of the tgf beta family of growth factor proteins it is vital for the differentiation of various types of cells in the body including immune cells microglia and normal growth and development these growth factors are highly conserved from c elegans all the way to humans tgfb1 exists as latency associated peptide and c-terminal growth factor chains that are non-covalently linked together lap inactivates the gf chain during its storage in the extracellular matrix tgf beta 1 is observed at approximately 45 kilodaltons topic 2 where is tgf beta 1 exp