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DocHub is a straightforward, cost-effective way to manage documents and improve workflows. It offers a wide range of features, from creation to editing, eSignature services, and web document building. The application can export your files in many formats while maintaining maximum safety and following the greatest information security criteria.
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After Western blot transfer is completed, the membrane undergoes a series of incubation and wash steps to prepare the membrane and probe it for your target protein. The first step, called blocking, coats the membrane surface with a protein or other molecule that is non-reactive to the antibody that will be used to probe for the target protein, or antigen. Nitrocellulose and PVDF membranes have a high protein-binding affinity, which is essential for successful Western blot analysis. The blocking step ensures that the antibody binds specifically to the antigen rather than non-specifically to the membrane surface, which can result in a high background signal that prevents detection of your protein of interest. The membrane is blocked with a buffer that is often comprised of Tris- or phosphate-buffered saline with Tween-20 detergent and up to 5% of a non-reactive protein such as bovine serum albumin, non-fat milk, gelatin or normal serum. The exact formulation and incubation period depends
