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welcome back letamp;#39;s kind of start where we kind of left off we had talked about cell cultures facts separating cells we talked about the use of antibodies monoclonal polyclonal okay we figured out a way to separate fractions of cells so cells by centrifugation okay centrifuge we rate zone amp;#39;l isil picnic all that we could get fractions and each fraction could contain an organelle and that led to protein right proteins proteins okay proteins and along the way we could do from centrifugation cell free systems we could then mimic what was going on inside cells so a cell-free system here okay cell free and then for these proteins clearly if we had found on in an organelle they must have some function right so weamp;#39;re very interested in function of proteins because that leads to functioning in the organelle and of course organelles are in the cell and there must be connections between organelles cell physiology and when we purified say mitochondria if youamp;#39