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in this tutorial we will learn how to perform a densitometric analysis for agar gel electrophoresis bands to quantify the intensities of agarose gel bands log on to the website as shown in the screen below click on download and then select the software that suits your operating system after download has been completed extract the file and find the image icon inside the folder for your convenience the image j icon can be exported to the desktop for this tutorial let us import an agarose gel image by drag and drop method onto image j in this example the gel image on the left hand side shows the amplified fibulin 1 bands consisting of a control sample one and sample two on the right hand side of the gel image the gel bands are of a housekeeping gene cap dh to perform a densitometric analysis of the bands of our interest first the image has to be converted into an 8 bit image the next step is to subtract the background or noise present in the agarose gel image the red arrow points to the f
