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milk itamp;#39;s good for your bones and your blots at least most of the time and itamp;#39;s not the calcium we care about when weamp;#39;re using milk in a blocking solution for a western blot itamp;#39;s the casein um and in case youamp;#39;re trying to blot for like phosphorylation you might want to use bsa instead of skim milk although because that casein um itamp;#39;s typically phosphorylated and then this can cause false signals and the point of using a blocking solution in a immunoblot like a western blot is to basically make the so that the background is lower onto one of these western blots what youamp;#39;re trying to do is you take proteins and you separate them by size and then you transfer them to a membrane this membrane is typically nitrocellulose or pvdf whatamp;#39;s gonna happen is that the proteins are gonna be like present in like different spots on here and theyamp;#39;ll be on like because you know where lanes are gonna be like lines but youamp;#39;re
