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western blotting is a fundamental biochemical technique that we can use to see if there are specific proteins in a sample and it builds off of a technique we talked about before sds page in which we used electricity to get proteins to move through a gel mesh and separate by size so with sds page you can separate the proteins by size and then you have these proteins locked in a gel so the question is what do you want to do next you can do something like a massey stain which will stain for all proteins and youamp;#39;ll get a bunch of bands but you wonamp;#39;t know what those bands are so western blotting allows you to see what those bands are what kind of you have to have you have to know what youamp;#39;re looking for you basically go fishing for specific fish using antibodies so iamp;#39;m going to give you an overview and then weamp;#39;re going to go in more detail in each of the steps so basically the idea is that you take proteins from different conditions so maybe you have
